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At high mutagen concentration, however, a carbon source present during treatment caused a 2- to 150-fold increase in revertant yield depending on the mutagen, the carbon source, and on the genetic background of the strain.

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Check daily updates and enjoy for free! In a strain lacking adenylate cyclase, acridine mutagenesis was not abolished under the experimental conditions used in this study, and the addition of cAMP during mutagenic treatment had no effect.

In mismatch repair-deficient strains, the presence of glucose during treatment with low mutagen concentration did not cause a decrease in revertant yield as drastic as in the wild type.

At low mutagen concentration, treatment in the presence of the carbon source to which the cells were adapted resulted in a decrease in revertant yield of several orders of magnitude compared with the yield in the absence of a carbon source.